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SRX20775608: ONT WGS of Elysia crispata: whole slug
1 OXFORD_NANOPORE (MinION) run: 1.6M spots, 3.7G bases, 3Gb downloads

Design: Genomic DNA was extracted from E. crispata whole bodies of wild adults starved for a minimum of 2 weeks. A CTAB or DNAzol protocol was used to generate high molecular weight DNA. Short DNA fragments less than 10 kbp were depleted using a SRE XS kit (Circulomics, Baltimore, MD) according to the manufacturers protocol. At least 1.9 g of high molecular weight genomic DNA was used as input for ONT LSK-109 library ligation kits and sequenced on an R9 MinION flow cell. Base calling of ONT reads was performed with Guppy v6.1.2 (Oxford Nanopore Technologies, Oxford, UK).
Submitted by: Purdue University
Study: Elysia crispata genome assembly and gene expression
show Abstracthide Abstract
Elysia crispata, like other sea slugs in the superorder Saccoglossa, has the remarkable ability to sequester chloroplasts from the algae they eat. In slug digestive cells, phagocytosed algal material is selectively degraded, leaving only stolen chloroplasts (called kleptoplasts) intact, which provide the slugs with energy and photosynthate. The goal of the project is to sequence and assemble the genome of E. crispata and to evaluate gene expression during slug development.
Sample:
SAMN35891709 • SRS18061534 • All experiments • All runs
Organism: Elysia crispata
Library:
Name: FAN31223_2
Instrument: MinION
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 1.6M spots, 3.7G bases, 3Gb
Run# of Spots# of BasesSizePublished
SRR250205111,600,0003.7G3Gb2023-07-16

ID:
28206394

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